|
Technical Performance
| Applications |
GS-FLX |
GS-FLX+ |
HiSeq2000 |
MiSeq |
HiSeq X Ten |
Pac Bio RS II |
| Performance |
Read number
(million) |
1.3 |
1.3 |
6000 |
12 |
12,000 |
~0.05/cell |
| Read length (bp) |
~400 |
> 500 |
1X50~2x100 |
1X36~2x150 |
2X150 |
~30,000 |
| Throughput per plate |
360~560Mb |
500~800Mb |
75GB~300GB |
440Mb~4.6Gb |
800GB~900GB |
~375 Mb/cell |
| Physical run type |
1/2,1/4,1/8 |
1/2,1/4,1/8 |
8 lanes |
1 lane |
8 lanes |
8 SMRT cells |
| Technology |
Running time
(Instrument +
Primary analysis1) |
10 hours+1day |
20 hours+2 days |
(up to2)
12day+3days |
(up to3) 27 hours
+2 hours |
3 days
+ 20 hours |
~3 hours/cell |
| Library Type |
multiplexing |
up to 12 MIDs4 |
up to 12 MIDs |
up to 348 IDX |
up to 348 IDX |
up to 2 IDX |
○ |
| Shotgun library |
 |
 |
|
|
|
|
| Mate Pair library |
3kb,8kb,20kb |
|
up to 10kb |
|
|
|
| Amplicon library |
|
|
|
|
|
|
1. Primary analysis means basic analysis only (raw data, base calling and assembly)
2. Paired read/run (2x100bp) base
3. Paired read/run (2x150bp) base
4. MIDs is Multiplex Identifiers defined by Roche Technical Bulletin (TCB No. 005-2009)
Recommended NGS platform for specified application
Available NGS platform for specified application
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MACROGEN USA