|
サンプル条件
- Sample Quality Check(QC)の際にsampleを2-3ulを使用します。
- Sample volumeは余裕をもってご準備下さい。
- 下記サンプル基準は2022年6月2日時点の基準です。
Illumina DNA Library
ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。
| Library Type |
Library Kit |
Type |
Total Amount |
Conc. |
DIN |
etc |
| Amplicon DNA library |
Metagenome Amplicon |
gDNA |
|
> 0.1ng/ul |
|
>10ul |
| Target amplicon DNA |
Amplicon |
|
> 0.1ng/ul |
|
>10ul |
| TruSeq Nano DNA (LMW) |
Amplicon etc. |
0.1ug |
|
|
|
| Targeted DNA library |
SureSelect V6 |
gDNA |
1.0ug |
|
>7.0 |
|
| SureSelect V6 (FFPE) |
gDNA |
0.2ug |
|
|
|
| SureSelect V5 |
gDNA |
1.0ug |
|
>7.0 |
|
| SureSelect V5 (FFPE) |
gDNA |
0.2ug |
|
|
|
| SureSelect Mouse |
gDNA |
1.0ug |
|
>7.0 |
|
| Whole Genome library |
TruSeq Nano DNA (350) |
gDNA |
0.1ug |
|
>7.0 |
|
| TruSeq Nano DNA (550) |
gDNA |
0.2ug |
|
>7.0 |
|
| TruSeq DNA PCR Free (350) |
gDNA |
1.0ug |
|
>7.0 |
|
| TruSeq DNA PCR Free (550) |
gDNA |
2.0ug |
|
>7.0 |
|
| Nextera DNA XT |
gDNA |
0.001ug |
|
>7.0 |
|
| Accel Methyl-Seq DNA library |
gDNA |
0.2ug |
|
>7.0 |
|
| TruSeq ChIP-seq library |
gDNA |
0.01ug |
|
>7.0 |
|
PacBio DNA Library
ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。
| Library Type |
Library Kit |
Type |
Total Amount |
Conc. |
Avg.Size (BP) |
etc |
| PacBio Sequel Library |
20kb SMRTbell Express |
gDNA |
8.1ug |
|
20,000 |
|
| 10kb SMRTbell Express |
gDNA |
8.1ug |
|
15,000 |
|
| PacBio Amplicon Library Construction (1kb to 5 kb) |
Amplicon etc. |
1.000ug |
|
|
|
| PacBio Sequel Microbial Library Construction |
gDNA |
4.1ug |
|
20,000 |
|
| PacBio SequelⅡ Library |
20kb SMRTbell Express |
gDNA |
8.1ug |
|
20,000 |
|
| 10kb SMRTbell Express |
gDNA |
8.1ug |
|
15,000 |
|
| HiFi (10 kb - 25 kb) |
gDNA |
8.1ug |
|
25,000 |
|
Illumina RNA Library
ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください
| Library Type |
Library Kit |
Type |
Total Amount |
Conc. |
RIN |
rRNA ratio |
DV200 |
etc |
| mRNA library |
TruSeq stranded mRNA |
mRNA |
0.1ug |
|
|
|
|
|
| TruSeq stranded mRNA |
Total RNA |
1.0ug |
|
7 |
1 |
|
|
| TruSeq stranded mRNA |
exosomal RNA |
0.01ug |
|
|
|
|
|
| SMART-Seq v4 (SMARTer Ultra low input RNA library) |
Total RNA |
0.01ug |
|
7 |
1 |
|
|
| SMARTer Stranded RNA library (PolyA) |
Total RNA |
0.05ug |
|
7 |
1 |
|
|
| TruSeq RNA Access |
FFPE RNA |
0.1ug |
|
|
|
50% |
|
| Small RNA library |
TruSeq Small RNA library |
Total RNA |
3.0ug |
|
7 |
1 |
|
|
| TruSeq Small RNA library |
exosomal RNA |
0.01ug |
|
|
|
|
|
| TruSeq Small RNA library |
small RNA |
0.1ug |
|
|
|
|
|
| SMARTer Small RNA library |
Total RNA |
0.05ug |
|
7 |
1 |
|
|
| SMARTer Small RNA library |
small RNA |
0.01ug |
|
|
|
|
|
| SMARTer Small RNA library |
exosomal RNA |
0.01ug |
|
|
|
|
|
| NEBNext Small RNA library |
Total RNA |
1.0ug |
|
7 |
1 |
|
|
| NEBNext Small RNA library |
small RNA |
0.1ug |
|
|
|
|
|
| NEBNext Small RNA library |
exosomal RNA |
0.01ug |
|
|
|
|
|
| Total RNA library |
TruSeq Stranded Total RNA with Ribo-Zero H/M/R |
Total RNA |
0.5ug |
|
7 |
1 |
|
|
| TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold |
Total RNA |
0.5ug |
|
7 |
1 |
|
|
| TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Globin |
Total RNA |
0.5ug |
|
7 |
1 |
|
|
| TruSeq Stranded Total RNA with Ribo-Zero Plant |
Total RNA |
0.5ug |
|
7 |
1 |
|
|
| TruSeq Stranded Total RNA (NEB Microbe) |
Total RNA |
0.5ug |
|
7 |
1 |
|
|
| SMARTer Stranded RNA library (RiboZero) |
Total RNA |
0.1ug |
|
7 |
1 |
|
|
PacBio RNA Library
ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください。
| Library Type |
Library Kit |
Type |
Total Amount |
RIN |
rRNA ratio |
DV200 |
etc |
| Sequel PacBio Library |
PacBio Sequel Iso-Seq Library Construction |
Total RNA |
0.600ug |
7 |
1 |
|
|
| PacBio Sequel Iso-Seq Library Construction (+4kb) |
Total RNA |
3.000ug |
7 |
1 |
|
|
Library Sequence (Run Only)
調製済みライブラリーをご送付いただく際は下記の濃度、液量をご準備ください。
| Platform |
Library Type |
Type |
Conc
(nM) |
Conc
(ng/ul) |
Size(bp)
From |
Size(bp)
To |
etc |
| NovaSeq6000 |
NGS library (Ready to Run) |
Library |
10 |
|
|
|
at least 20ul |
| MiSeq |
NGS library (Ready to Run) |
Library |
5 |
|
|
|
at least 20ul |
| HiSeq2500 |
NGS library (Ready to Run) |
Library |
5 |
|
|
|
at least 20ul |
| HiSeqX |
NGS library (Ready to Run) |
Library |
5 |
|
|
|
at least 20ul |
|
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