サンプル条件｜マクロジェン・ジャパン

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サンプル条件

サンプル条件

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type	Library Kit	Type	Total Amount	Conc.	DIN	etc
Amplicon DNA library	Metagenome Amplicon	gDNA		> 0.1ng/ul		>10ul
Amplicon DNA library	Target amplicon DNA	Amplicon		> 0.1ng/ul		>10ul
Targeted DNA library	SureSelect V6	gDNA	1.000ug	>20ng/ul
	SureSelect V6 (FFPE)	gDNA	0.200ug	>10ng/ul
	SureSelect V5	gDNA	1.000ug
	SureSelect V5 (FFPE)	gDNA	0.200ug
	SureSelect Mouse	gDNA	1.000ug
Whole Genome library	TruSeq Nano DNA (350)	gDNA	0.100ug	>10ng/ul
	TruSeq Nano DNA (550)	gDNA	0.200ug	>20ng/ul
	TruSeq Nano DNA (LMW)	Amplicon etc.	0.100ug
	TruSeq DNA PCR Free (350)	gDNA	1.000ug	>20ng/ul
	TruSeq DNA PCR Free (550)	gDNA	2.000ug	>20ng/ul
	Nextera DNA XT	gDNA	0.001ug
	Accel Methyl-Seq DNA library	gDNA	0.200ug
	TruSeq ChIP-seq library	gDNA	0.010ug
	Nextera mate pair library (gel plus)	gDNA	4.000ug

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type	Library Kit	Type	Total Amount	Conc.	DIN	etc
PacBio RSII Library	20kb SMRTbell TPK	gDNA	8.000ug		>9.0
	10kb SMRTbell TPK	gDNA	6.000ug		>9.0
	PacBio Amplicon Library Construction (1kb to 5 kb)	Amplicon etc.	1.000ug
PacBio Sequel Library	20kb SMRTbell Express	gDNA	8.000ug		>9.0
	PacBio Amplicon Library Construction (1kb to 5 kb)	Amplicon etc.	1.000ug
	PacBio Sequel Microbial Library Construction	gDNA	3.000ug		>9.0
PacBio SequelⅡ Library	Large insert (>15kb size-selection cut off)	gDNA	8.000ug		>9.0
PacBio SequelⅡ Library	HiFi (10 kb - 25 kb)	gDNA	16.000ug		>9.0

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください

Library Type	Library Kit	Type	Total Amount	Conc.	RIN	rRNA ratio	DV200	etc
mRNA library	TruSeq stranded mRNA	mRNA	0.100ug	>10ng/ul
	TruSeq stranded mRNA	Total RNA	1.000ug	>20ng/ul	7	1
	TruSeq stranded mRNA	exosomal RNA	0.010ug
	SMARTer Ultra low input RNA library	Total RNA	0.010ug		7	1
	SMARTer Stranded RNA library	Total RNA	0.050ug		7	1
	TruSeq RNA Access	FFPE RNA	0.100ug				50%
Small RNA library	TruSeq Small RNA library	Total RNA	3.000ug		7	1
	TruSeq Small RNA library	exosomal RNA	0.010ug
	TruSeq Small RNA library	small RNA	0.100ug
	SMARTer Small RNA library	Total RNA	0.050ug		7	1
	SMARTer Small RNA library	small RNA	0.010ug
	SMARTer Small RNA library	exosomal RNA	0.010ug
	NEBNext Small RNA library	Total RNA	3.000ug		7	1
	NEBNext Small RNA library	small RNA	0.100ug
	NEBNext Small RNA library	exosomal RNA	0.010ug
Total RNA library	TruSeq Stranded Total RNA with Ribo-Zero H/M/R	Total RNA	0.500ug		7	1
	TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold	Total RNA	0.500ug		7	1
	TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Globin	Total RNA	0.500ug		7	1
	TruSeq Stranded Total RNA with Ribo-Zero Plant	Total RNA	0.500ug		7	1
	TruSeq Stranded Total RNA (NEB Microbe)	Total RNA	1.000ug		7	1

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください。

Library Type	Library Kit	Type	Total Amount	RIN	rRNA ratio	DV200	etc
PacBio RSII Library	PacBio RSII Iso-Seq Library Construction	Total RNA	2.000ug	7	1
Sequel PacBio Library	PacBio Sequel Iso-Seq Library Construction	Total RNA	0.600ug	7	1
Sequel PacBio Library	PacBio Sequel Iso-Seq Library Construction (+4kb)	Total RNA	3.000ug	7	1

調製済みライブラリーをご送付いただく際は下記の濃度、液量をご準備ください。

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