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DNAの品質
- コンタミがなく，分解していない2本鎖DNA
- ゲルまたはカラムで精製したDNA
- A260/280の割合が1.8-2.0
RNAの品質
- RIN(RNA Integrity Number)値が7以上
- 28S rRNAバンドの厚さが18S rRNAバンドの約2倍
サンプル量の測定方法（弊社推奨）
- DNAサンプルは、Victor 3 fluorometryによるPicoGreen法
- RNAサンプルは、2100 BioanalyzerまたはTapeStationを使用
GS FLX
| Library Type |
Sample Type |
conc.(ng/ul) |
vol.(ul)* |
total amount(ug) |
Shotgun library
(Rapid Shotgun) |
High Molecular Weight
DNA ( >1.5kb) |
>50 |
>20 |
1 |
PCR product , Low molecular
weight |
>10 |
>20 |
>0.2 |
| Mate Pair library |
3kb insert |
>100 |
>50 |
>5 |
| 8kb insert |
>100 |
>150 |
>15 |
| 20kb insert |
>100 |
>300 |
>30 |
| Rapid cDNA library |
purified mRNA |
>11 |
>19 |
>0.2 |
| Total RNA |
>250 |
>200 |
>50 |
* minimum concentration should maintain constantly regardless of volume condition.
HiSeq / MiSeq
| Library Type |
Sample Type |
conc.(ng/ul) |
vol.(ul)* |
total amount(ug) |
| Shotgun (TruSeq) |
DNA |
>20 |
>50 |
>1 |
| Mate Pair Library (up to 5kb) |
DNA |
>100 |
>100 |
>10 |
| mRNA Library |
total RNA |
>20 |
>50 |
>4 |
| mRNA |
>10 |
>10 |
>0.1 |
| small RNA Library |
total RNA |
- |
- |
>3 |
| ChIP seq library* |
- |
- |
- |
>10ng |
| Nextera library |
DNA |
2.5 |
>20 |
>50ng |
| Lucigen library |
DNA |
10~20ug / ul |
>10 |
100~200 |
* 200~300bp size sonicated samples are desired, and Control DNA must be provided. If you don’t want sequence the control DNA, please contact in advance.
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MACROGEN USA