サンプル条件｜マクロジェン・ジャパン

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サンプル条件

サンプル条件

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type	Library Kit	Type	Total Amount	Conc.	DIN	etc
Amplicon DNA library	Metagenome Amplicon	gDNA		> 0.1ng/ul		>10ul
Amplicon DNA library	Target amplicon DNA	Amplicon		> 0.1ng/ul		>10ul
Targeted DNA library	SureSelect V6	gDNA	1.000ug	>50ng/ul
	SureSelect V6 (FFPE)	gDNA	0.200ug	>10ng/ul
	SureSelect V5	gDNA	1.000ug	>50ng/ul
	SureSelect V5 (FFPE)	gDNA	0.200ug	>10ng/ul
	SureSelect Mouse	gDNA	1.000ug	>50ng/ul
Whole Genome library	TruSeq Nano DNA (350)	gDNA	0.100ug	>10ng/ul
	TruSeq Nano DNA (550)	gDNA	0.200ug	>20ng/ul
	TruSeq Nano DNA (LMW)	Amplicon etc.	0.100ug
	TruSeq DNA PCR Free (350)	gDNA	1.000ug	>20ng/ul
	TruSeq DNA PCR Free (550)	gDNA	2.000ug	>20ng/ul
	Nextera DNA XT	gDNA	0.001ug	>0.2ng/ul
	Accel Methyl-Seq DNA library	gDNA	0.200ug	>10ng/ul
	TruSeq ChIP-seq library	gDNA	0.010ug	>1ng/ul
	Chromium Genome library	gDNA	1.000ug	>50ng/ul	>7.0
	Nextera mate pair library (gel plus)	gDNA	4.000ug	>100ng/ul

ライブラリー調製に使用するKitをご確認の上、下記条件のDNAをご準備ください。

Library Type	Library Kit	Type	Total Amount	Conc.	DIN	etc
PacBio RSII Library	20kb SMRTbell TPK	gDNA	8.000ug	>50ng/ul
	10kb SMRTbell TPK	gDNA	6.000ug	>50ng/ul
	PacBio Amplicon Library Construction (1kb to 5 kb)	Amplicon etc.	1.000ug	>50ng/ul
PacBio Sequel Library	20kb SMRTbell Express	gDNA	8.000ug	>50ng/ul

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください

Library Type	Library Kit	Type	Total Amount	Conc.	RIN	rRNA ratio	DV200	etc
mRNA library	TruSeq stranded mRNA	mRNA	0.100ug	>10ng/ul
	TruSeq stranded mRNA	Total RNA	1.000ug	>20ng/ul	7	1
	TruSeq stranded mRNA	exosomal RNA	0.010ug
	SMARTer Ultra low input RNA library	Total RNA	0.010ug	>2ng/ul	7	1
	SMARTer Stranded RNA library	Total RNA	0.050ug	>2ng/ul	7	1
	TruSeq RNA Access	FFPE RNA	0.100ug				50%
Small RNA library	TruSeq Small RNA library	Total RNA	3.000ug		7	1
	TruSeq Small RNA library	exosomal RNA	0.010ug
	TruSeq Small RNA library	small RNA	0.100ug
	SMARTer Small RNA library	Total RNA	0.050ug	>10ng/ul	7	1
	SMARTer Small RNA library	small RNA	0.010ug
	SMARTer Small RNA library	exosomal RNA	0.010ug
	NEBNext Small RNA library	Total RNA	3.000ug		7	1
	NEBNext Small RNA library	small RNA	0.100ug
	NEBNext Small RNA library	exosomal RNA	0.010ug
Total RNA library	TruSeq Stranded Total RNA with Ribo-Zero H/M/R	Total RNA	0.500ug	>20ng/ul	7	1
	TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold	Total RNA	0.500ug	>20ng/ul	7	1
	TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Globin	Total RNA	0.500ug	>20ng/ul	7	1
	TruSeq Stranded Total RNA with Ribo-Zero Plant	Total RNA	0.500ug	>20ng/ul	7	1
	TruSeq Stranded Total RNA (NEB Microbe)	Total RNA	1.000ug		7	1

ライブラリー調製に使用するKitをご確認の上、下記条件のRNAをご準備ください。

Library Type	Library Kit	Type	Total Amount	Conc.	RIN	rRNA ratio	DV200	etc
PacBio RSII Library	PacBio RSII Iso-Seq Library Construction	Total RNA	2.000ug	>100ng/ul	7	1
Sequel PacBio Library	PacBio Sequel Iso-Seq Library Construction	Total RNA	2.000ug	>100ng/ul	7	1
Sequel PacBio Library	PacBio Sequel Iso-Seq Library Construction (+4kb)	Total RNA	3.000ug	>100ng/ul	7	1

調製済みライブラリーをご送付いただく際は下記の濃度、液量をご準備ください。

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